Journal: Journal of molecular endocrinology
Article Title: clc-2c is regulated by salinity, prolactin and extracellular osmolality in tilapia gill
doi: 10.1530/JME-17-0144
Figure Lengend Snippet: Effects of hypophysectomy (Hx) and ovine Prl (oPrl) on plasma osmolality (A) and Cl− (B) and branchial gene expression of ncc2 (C), clc-2c (D) and clc-3 (E). Mean ± SEM (n = 8–9). Gene expression is presented as a fold-change from the saline-injected sham group (open bars). While held in brackish water (12‰), fish received three intraperitoneal injections of oPrl (5 μg/g body weight) (solid bars) over 5 days (see ‘Materials and methods’). Sham-operated (open bars) and Hx fish (shaded bars) received saline injections. Means not sharing the same letter are significantly different (one-way ANOVA, Tukey’s HSD test, P < 0.05).
Article Snippet: Plasma was separated by centrifugation for measurement of plasma osmolality using a vapor pressure osmometer (Wescor 5100C, Logan, UT) and plasma Cl − by the silver titration method using a Buchler-Cotlove digital chloridometer (Labconco, Kansas City, MO).
Techniques: Clinical Proteomics, Gene Expression, Saline, Injection